TM与VEGF在宫颈鳞癌组织中的表达和意义

Expression of TM and VEGF in cervical squamous carcinoma tissues and their significance

目的了解血栓调节蛋白与血管内皮生长因子在宫颈鳞癌组织中的表达情况及其与病变的临床分期、组织学分级和淋巴结转移等方面的关系,并分析血管内皮生长因子与血栓调节蛋白的相关性。方法采用免疫组织化学方法分别检测血栓调节蛋白与血管内皮生长因子在34例宫颈鳞癌、34例宫颈上皮内瘤变、8例慢性宫颈炎组织中的表达,并进行分析。结果血栓调节蛋白在宫颈鳞癌、宫颈上皮内瘤变、宫颈炎组织中的表达阳性率分别为17.65%、50%、62.5%,在宫颈上皮内瘤变Ⅲ级、Ⅱ级、Ⅰ级组织中的表达阳性率分别为23.5%、70%、85.7%,均呈逐渐升高趋势,且有显著性差异(P<0.05),而在宫颈鳞癌组,血栓调节蛋白的表达与临床分期、组织学分级和淋巴结转移无关(P>0.05)。血管内皮生长因子在宫颈鳞癌、宫颈上皮内瘤变、宫颈炎组织中的表达阳性率逐渐降低,分别为70.6%、44%、25%,有显著性差异(P<0.05),血管内皮生长因子的表达与宫颈鳞癌临床分期、组织学分级和淋巴结转移以及宫颈上皮内瘤变分级无关(P>0.05)。血栓调节蛋白与血管内皮生长因子在宫颈鳞癌中的表达亦无明显相关性(P>0.05)。结论血栓调节蛋白与血管内皮生长因子均可能作为宫颈鳞癌的肿瘤标志物,对肿瘤的发生分别起抑制和促进作用,在宫颈鳞癌的筛查中,血栓调节蛋白较血管内皮生长因子更有临床意义。

Objective To investigate expression of thrombomodulin （TM） and vascular endothelial growth factor （VEGF） in cervical squamoas carcinoma and relationship of expression of TM and VEGF with the latest stages of the tumor worked-up by International Federation of Gynecology and Obstetrics, histological grade and lymphatic metastasis, and correlation between TM and VEGF. Methods The expression of TM and VEGF was detected by immunohistoehemistry method in sample tissues of 34 cases of cervical carcinoma, 34 cases of cervical intraepithelial neoplasia （CIN） and 8 cases of chronic cervicitis. Results The positive expression rate of TM in cervical carcinoma,CIN and chronic cervieitis wasl7.65%, 50% and 62. 5%, respectively. From CIN Ⅲ grade to CIN Ⅰ grade,the positive expression rate of TM was 23.5%, 70% , 85.7% in order, there was an increasing tendency and significant difference（ P 〈 0.05 ）. The strong expression rate of VEGF in cervical carcinoma , CIN and chronic eervicitis was70.6% , 44% and 25%, respectively and there was a significantly decreasing tendency （ P 〈 0.05 ） while in that of VEGF in all grades of CIN there was no statistically significant difference （ P 〉 0.05 ）. Neither TM nor VEGF had close relationship with clinical stage , histological grade and lymphatic metastasis（ P 〉 0.05 ）. No correlation between expressions of TM and VEGF was observed in cervical carcinoma（ P 〉 0.05 ）. Conclusion Both TM and VEGF can be regarded as tumor markers of cervical carcinoma. TM inhibits while VEGF promotes development of the malignancy. For screening of cervical carcinoma, TM seems to be of more clinical significance than VEGF.