livin靶向RNA干扰质粒的鉴定

Identification of livin-targeting RNA-interfering plasmid

目的:验证liv in靶向RNA干扰重组质粒是否构建成功并成功转化入大肠杆菌。方法:从已经转化的大肠杆菌中提取质粒,并经限制性内切酶酶切、A grose电泳,测序。结论:酶切图谱分析,测序结果显示重组质粒已成功构建并转化入大肠杆菌。

Objective： To verify whether the livin--targeting RNA -- interfering recombinant plasmids are successfully constructed and are transformed into E. coli. Methods：The plasmids were extracted from transformed E. coli, and then electrophoresis was performed with agrose gel after being digested by the restriction enzyme, and sequence analysis was made. Conclusion： The recombinant plasmids have been successfully constructed and transformed into E. coll.