家蚕血细胞在体外培养过程中的变化

Changes of Hemocytes From the Silkworm (Bombyx mori) in vitro Culture

为建立家蚕血细胞系,以家蚕血淋巴为材料,对其进行了长达1年多的原代培养,并从形态学上进行了观察与分类。详细记录了培养过程中出现的多种细胞或组织的分化或转分化现象,发现有2种家蚕血细胞在体外培养条件下有一定的增殖能力,暗示家蚕血细胞体外建系具有可行性。

In order to establish hemocyte line, the hemolymph from silkworm larvae had been primary cultured about one year. Based on morphological observation and taxonomy, the differentation and transdifferentation of cells and tissues were recorded in details. It was found that two kinds of hemocytes had proliferation activity, which implied that it is likely to establish cell line from Bombyx moil hemocytes in vitro.