摘要
首次研究分析了灰葡萄孢霉菌群体中旁路氧化与菌丝生长对嘧菌酯敏感性之间的关系。结果表明,旁路氧化酶的专化性抑制剂水杨肟酸(SHAM)对不同菌株菌丝生长的影响不同:50μg/mLSHAM对采自浙江、江苏、山东三地的142株灰葡萄孢霉菌菌丝生长的抑制率在-29.03%-48.33%之间,平均为10.49%。SHAM处理对嘧菌酯抑制菌丝生长的活性表现出明显的协同增效作用:无SHAM时,嘧菌酯对上述群体菌丝生长的EC50(EC50Q)值在0.20~51.48μg/mL之间,平均为6.56μg/mL;当SHAM浓度为50μg/mL时,EC50[EC50(Q+s)]值在0.04—5.25μg/mL之间,平均为2.21μg/mL。旁路氧化的相对贡献值F[F=EC50Q/EC50(Q+S)]最低为0.17,最高为24.40,平均为3.19。另外,少数在没有SHAM存在时对嘧菌酯表现“耐药性”的菌株在SHAM存在下对嘧菌酯也很敏感。
The relationship between alternative respiration and sensitivity to azoxystrobin during the stage of mycelial growth in B. cinerea population was analysed. The results showed that salicylhydroxamic acid (SHAM), a specific inhibitor of alternative oxidase, had different impact on growth rate of different isolates. Inhibition percentage of SHAM at a dose of 50 μg/mL to the tested 142 isolates collected from Zhejiang, Jiangsu and Shandong province ranged from -29.03% to 48.33% ,with an average value of 10. 49%. Meanwhile, SHAM expressed a notable synergistic interaction with azoxystrobin against B. cinerea. In the absence of SHAM, medium effective concentrations (EC50 Q) inhibiting growth rate of the tested population by azoxystrobin ranged from 0.20 to 51.48 μg/mL,with an average value of 6.56 μg/mL. In the presence of 50 μg/mL SHAM, the EC50[ (EC50(Q +S) ] ranged from 0.04 to 5.25 μg/mL,with an average value of 2.21 μg/mL. The factor value [ F = EC50Q/EC50 (Q + S ) ] for the impact of alternative respiration on sensitivity to azoxystrobin ranged from 0.17 to 24.40, with an average value of 3.19. Moreover, some ‘tolerant' isolates in the absence of SHAM were also senitive to azoxystrobin when SHAM presented.
出处
《农药学学报》
CAS
CSCD
2006年第4期306-312,共7页
Chinese Journal of Pesticide Science
基金
第38批中国博士后科学基金(2005038641)资助