鸡胚细胞的微载体培养

The Cluture of Chicken Embyro Fibroblast Cells on Microcarriers

国外有许多利用微载体培养各种贴壁细胞的报道。鸡胚细胞(CEF cells)是最早用于体外培养的细胞之一,用它可生产多种鸡的疫苗,如法氏囊、新城疫、马立克疫苗等。我国生产上多采用滚瓶法,近年来才有微载体法培养的探索。要进行细胞的大规模培养,转瓶是有效的模拟。

The culture of primary chicken embyro fibroblast (CEF) cellsat on microcar-riers in spinner bottles to produce infactious bursal disease virse (IBDV) has been investigated. It was found that there exisited significant difference in the plating efficiency and growth rate of CEF cell at different inoculation density on GT-2 microcarriers. Therefore, the inoculation density for the CEF cell culture was optimized, and the plating process of CEF cells on GT-2 microcarriers was studied. While the additive microcarriers were fed during the culture, the cell density and IBDV yield increased. All the above experimental results have laid the foundation for high density culture of CEF cells and process scale-up.