应用FIA型微生物传感器测定谷氨酸含量的研究

The Study of a FIA Microbial Probe System-a Microbial Sensor for the Measurement of Glutamate

目前应用酶或微生物细胞作为分子识别元件构建生物传感器测定谷氨酸含量的研究引起了广泛的兴趣,并陆续有实例报道。在这些报道中人们依据不同的酶催反应选择相应的离子选择性电极,如CO_2电极、NH_4^+电极等,而测量对象有直接的测定谷氨酸,也有测定谷氨酸单钠的间接测定法。本文选用了可固定大量细胞的固定化细胞柱与流动注入法相结合的测量方法。

A FIA microbial sensor system combined with an immobilized E. colt cell column, a CO2 gas sensitive selectrode was made for measurement of glutamate. The system working range (concentration of Glu)is from 400 to 2000 mg/L and it has high specificity for glutamate (among the amino acid) except L-arginine and L-glutamine. The measuring time for each sample is about 20min. The half life of the immobilized E. colt is more than 4 weeks. A mathematic model was constructed for a immoblized cell column. The calculation method of the measurement value and its results are given in a L-gluta-mate microbial sensor process.