烷基聚氧乙烯醚硫酸钠对肌酸激酶的变性及复性

THE DENATURATION AND RENATURATION OF CREATINE KINASE BY SODIUM DODECYLPOLYOXYETHLENE SULFATE

系统的研究了十二烷基聚氧乙烯醚硫酸钠Ｃ１２Ｈ２５（ＯＣ２Ｈ４）ｎＳＯ４Ｎａ（ｎ＝０，１，３，５，７；记为Ｃ１２ＥｎＳ）对肌酸激酶的活力和构象的影响。结果表明：随着氧乙烯基个数（ｎ）的增加，Ｃ１２ＥｎＳ对Ｃ．Ｋ．的活力破坏逐步减小；Ｃ１２ＥｎＳ引起Ｃ．Ｋ活力的丧失明显早于可测构象的变化。ＣＫ经Ｃ１２ＥｎＳ变性后，稀释时即可完全复性。Ｃ１２Ｅ７Ｓ不仅对Ｃ．Ｋ的变性能力强（１０ｍＭ就使Ｃ．Ｋ完全失活）；而且在１０－９５℃的温度范围内，能阻止Ｃ．Ｋ聚集沉淀。Ｃ１２ＥｎＳ是Ｃ．Ｋ的高效可逆变性剂。

The denaturation and renaturation of creating kinase,by sodium dodecylpolyoxyethlene sulfate C12H25(OC2H4)nSO4Na (n=0,3,5,7,C12EnS),were studied in detail.The results showed that the effect of denaturation of C12EnS decreased with increasing of n.The inactiration of CK caused by C12EnS is much earlier than changes in conformation detected.The denaturated CK,by C12EnS,can be renaturated when the denathrant is diluted. C12EnS is a high efficiency denaturant(10mmol/L can incativate CK completely)C12E7S can prevent CK from preciptating in solution(in the range of 10-95 ℃.