睾酮在北方山溪鲵精巢发育周期中的分布

Localization of testosterone in the testis of the stream salamander Batrachuperus tibetanus during the annual reproductive cycle

用免疫组织化学方法检测了睾酮（testosterone，T）在北方山溪鲵（Batrachuperus tibetanus）精巢发育周期中的变化。结果表明，在北方山溪鲵的精巢中，4月份T定位在次级精原细胞的胞质中;5月份T定位在次级精原细胞的胞核中，6～7月，精母细胞和精子细胞核中呈T阳性反应;8月份，精巢的非成熟区和成熟区散在地出现T的弱阳性位点，9～10月，精巢非成熟区的精原细胞胞质及成熟区精子中有较强的T阳性反应;11月，精原细胞的胞质及精子中的阳性反应减弱。在山溪鲵的生殖周期中，4～11月精巢中始终呈现T阳性反应，而且T的分布呈现出一定的规律性。在4～5月和9～10月，精巢间质细胞和支持细胞中也有T阳性反应。上述结果说明．在山溪鲵精巢中，T对生殖细胞增殖及精子形成起调控作用。

Location of testosterone （T） in testis of Batrachuperus tibetanus was examined by immunohistochemistry. The results were as follows-testosterone was presented in the cytoplasm ot spermatogonia （SPG） in April. It was detected in the nuclear of SPG in May when SPG would become primary spermatoeyte （ISPC）. During Jun-Jul,T positive reactivity was also detected in the nuclear of spermatocyte （SPC） and spermatid （SPD）. In Aug,there were T slight positive reaction dots scattered in the immature region and mature region. In Sep-Oct, T was presented in the cytoplasm of SPG in immature region and spermatozoa （SPZ） in mature region. T positive reactivity weakened in the cytoplasm of SPG and SPZ in Nov. During the annual reproductive cycle,T positive location always presented in the certain part in the testis. T was also detected in Leydig cells （LC） and Sertoli cells （SC） of the testis during Apt-May and Sep-Oet. The result shows that testosterone disperses periodically and regularly in testis of Batrachuperus tibetanus and it plays an regulative role in proliferation of germ cell and spermatogenesis.