PRV FB弱毒株与FA株gE,gI基因的同源性研究

Study on the homology between PRV FB low virulent strain and gE and gI of PA strain

用PCR方法扩增了PRVFB弱毒株和PRVFA野毒株的gE,gI基因,对FB弱毒株的gE,gI基因进行克隆和测序,并将其与GenBank中收录的PRVFA的gE,gI基因进行了比较。结果发现,PRVFB和PRVFA株的gE基因有2处碱基发生了点突变,同源性为99%;gI基因有7处碱基发生了突变,其中1处发生了3个碱基的插入突变,同源性为98%。表明PRVFB在传代过程中发生了遗传性变异。

The gI and gE gene of PRV FB （low virulent strain） and PRV FA （high virulent strata） were amplified by PCR,then the gI and gE gene of PRV FB were cloned and sequenced. Blasted with gE and gI gene of PRV FA from GenBank ,2 bases in gE were found to have point mutation,and the homology was over 99 %Meanwhile, 7 bases in gI resulted in point mutation and 3 bases were inserted ,and the homology.was over 98~%. These mutation revealed PRV FB occur in heritage mutation and deserve further research.