抗大肠杆菌O_(157)鸡卵黄抗体的酶联免疫吸附试验

Indirect ELISA detection of IgY specific on E.coli O_(157)

以灭活大肠杆菌（E．coli）O157为抗原免疫产蛋鸡获得抗E．coli O157卵黄抗体（IgY），建立了该抗体的间接酶联免疫吸附试验（ELISA）检测方法。并研究了包被温度和时间及不同卵黄前处理方法对ELISA检测的影响，所获得的最佳检测条件为：以浓度为10^8～10^9／mL的灭活E．coliO157，为抗原进行包被，4℃包被19h，水稀释法对卵黄进行前处理，然后检测其中的IgY效价。该方法简便可靠，适于对大肠杆菌特异性抗体IgY的动态检测和批量检测。

Chicken was immunized with E. coli O157 as antigen,which produced a large amount of immunoglobulin of high titer in egg yolk,also called IgY. Indirect ELISA detection of IgY specific on E. coli O157 was studied. The parameters were as follows：polystyrene chloride microtitration plates were coated with E. coli O157 antigen at the amount of 10^8-10^9/mL,19 h at 4 C. The working concentration of HRP-labelled rabbit antichicken IgG was 1 ： 320. Egg yolk pretreated with water-dilution improved the performance of ELISA detection. The ELISA system is dependable and good for the assay and surveillance of E. coli O157 specific IgY.