玉米叶片叶绿素快速浸提方法研究

Fast-soaking methods of chlorophyll from maize leaf

以玉米叶片为材料,研究了体积分数80%丙酮、体积分数95%乙醇、N,N-二甲基甲酰胺(DMF)、乙醇-丙酮(体积比1∶1)混合液4种浸提液,不同光温条件和不同样品预处理方式对叶绿素浸提的影响。结果表明,浸提液以乙醇-丙酮混合液效果最好,避光和50℃的提取温度有利于叶绿素提取;样品冷冻处理不利于叶绿素a的提取,而有利于叶绿素b的提取,总叶绿素以鲜样提取为宜。在大样本试验中浸提叶片叶绿素较适宜的方法是:将新鲜(或冷冻)玉米叶片放入50℃丙酮-乙醇混合液(体积比1∶1)中避光提取1.5h。

Effect of four organic solvents,including acetone （90 % ）, alcohol （95 % ）, N, N-dimethylformanmide and mixture solution of alcohol and acetone （V/V= 1 ： 1） under the different conditions of temperature and light and pretreatments of samples,was evaluated on the extraction efficiency of chlorophyll from maize leaves. The results showed that mixture solution of acetone and alcohol （V/V=1 ： 1） was the best soaking solvent ;light-screening and proper higher temperature （50℃ ） were preferable for the extraction of chlorophyll;sample-freezing was favorable to the extraction of chlorophyll a,but unfavorable to chlorophyll b. Considering total chlorophyll,fresh sample was preferred. In conclusion,the paper proposed that the chlorophyll fast-soaking method,i, e. mixture solution of alcohol and acetone （V/V= 1 ： 1） at the temperature of 50 ℃ for 1.5 h,meet the need of batch measurement of chlorophyll.