骆驼蓬醇提物抑菌活性的初步研究

Antifungal activity of ethanol extrtacts from Peganum harmala L.

采用抑制菌丝生长法和毒力测定法,研究了骆驼蓬地上部醇提物的石油醚、氯仿、乙酸乙酯、正丁醇和水5个极性部位对南瓜枯萎病菌(Fusariumbulbigenum)、西瓜枯萎病菌(Fusariumoxysporumf.niveum)、辣椒疫霉病菌(Phytohthoracapsici)、苹果腐烂病菌(Valsamali)和番茄早疫病菌(Alternariasolani)等5种常见植物病原菌的抑制作用。结果表明,氯仿部分、乙酸乙酯部分和正丁醇部分对供试植物病原菌均有抑制作用。其中氯仿部分的抑菌效果较好,当质量浓度为15mg/mL时,其对5种植物病原菌的抑制率均在83%以上;15mg/mL乙酸乙酯部分和正丁醇部分分别对番茄早疫病菌和苹果腐烂病菌有强烈且稳定的抑制作用,抑制率达100.00%,正丁醇部分对这两种菌丝生长抑制毒力最好,EC50分别为0.090和0.279mg/mL。

The hypha growth-inhibiting method and the virulence determination method were used to investigate the inhibition effects of the petroleum ether fraction ,chloroform fraction ,ethyl acetate fraction, n-butanol fraction and water fraction in the alcoholic extracts from the aboveground of Peganurn harrnala L. on the five types of popular plant pathogenic fungi,Alternaria solani,Phytohthora capsici,Fusariurn bulbigenurn,Fusariurn oxysporurn f. niveurn and Valsa mall. The results showed that chloroform fraction ,n-butanol fraction,and ethylacetate fraction can inhibit the growth of five types of popular plant pathogenic fungi. The inhibition effect of chloroform fraction was the most potent, with the inhibition rate about more than 83% to 5 plant pathogenic fungi under the concentration of 15 mg/mL. Under the concentration of 15 mg/mL,the ethyl acetate fraction and n-butanol fraction thoroughly inhibited the growth of Alternaria solani and Valsa rnali,with the inhibition rate up to 100%. The n-butanol fraction showed the highest potent virulence,with EC50 to Alternaria solani and Valsa mall about 0.09 and 0. 279 mg/mL respectively.