紫花地丁抑菌活性成分的研究初报

Antibacterial constituent of Viola yedoensis Makino

采用活性跟踪法从紫花地丁的氯仿萃取物中分离得到1个抗菌活性成分(化合物1),并以停乳链球菌、金黄色葡萄球菌、大肠杆菌、无乳链球菌、乳房链球菌和沙门氏菌为供试菌,对紫花地丁的抑菌活性及其活性成分进行了初步研究。结果表明,化合物1初步鉴定为黄酮类化合物;紫花地丁的主要活性成分位于甲醇-水(V(甲醇)∶V(蒸馏水)=1∶1)萃取物和氯仿萃取物中;化合物1对以上6种供试菌的最小抑菌浓度依次为0.039,0.078,0.156,0.313,0.625和1.25mg/mL;对金黄色葡萄球菌、无乳链球菌和乳房链球菌的最小杀菌浓度≤2.5mg/mL,对停乳链球菌、沙门氏菌和大肠杆菌的最小杀菌浓度>2.5mg/mL。

Antibacterial activity of Viola yedoensis Makino and its antibacterial constituents were studied in this paper against S. aureas,S, agalactiae,S, uberis,S, dysagalactiae,E, coli and Salmonella in vitro. One antibacterial constituent （compound 1） from the chloroform extract was obtained with tracing-antibacterial activity method. The results showed that compound 1 was preliminarily identified as flavone;that main antibacterial comstituents in V. yedoensis Makino existed in chloroform phase and ethanol-water （1 ： 1） phase,that minimal inhibitory concentrations （MIC） of compound 1 were 0. 039,0. 078,0. 156,0. 313, 0. 625 and 1.25 mg/mL respectively against S. dysagalactiae , S. aureas,E, coli ,S. agalactiae ,S. uberis and Salmonella,and minimal bactericidal concentrations （MBC） were ≤ 2. 5 mg/mL against S. aureas,S, agalactiae and S. uberis,and 〉2.5 mg/mL against S. dysagalactiae,E, coli and Salmonella.