苏云金芽孢杆菌固态发酵条件的优化

Optimization of Solid Fermentation Conditions for Bacillus Thuringiensis

利用农产品下脚料作培养基，对菌株Bt HD-1的固态发酵工艺条件进行了优化，确定其发酵的最优条件为：初始含水量60％，pH值7．5～8．0，种子液接种量15％。种龄7h，固态发酵时间42h。测定表明最佳发酵条件下产品的毒力效价可稳定在18000IU·mg^-1，超过了国内文献报道的最高发酵水平，同时发酵时间缩短了8h。

Using agricultural product waste as medium, the solid fermentation process of Bt HD-1 was studied. Optimum conditions of solid fermentation of Bt HD-1 were determined as follows： initial moisture content 60%, pH value 7.5-8.0, inoculum size 15%, seed age 7 h, fermentation time 42 h. The bioassay efficacy of the product could be 18000 IU · mg^-1 under the optimum fermentation conditions, which exceeded the best fermentation level with less hours.