摘要
以Ｔ７为启动子构建了蜘蛛杀虫肽基因的表达质粒ｐＴＨＩ９，转化大肠杆菌ＢＡＬ３１，在其对数生长期加入１ｍｍｏｌ／ＬＩＰＴＧ能诱导杀虫肽的产生，表达产物占菌体总蛋白的１３．７５％，并且表达的杀虫肽具有功能活性．
The synthesized gene encoding insecticidal peptide was inserted into expression vector pET11c and controlled by T7 promoter. The recombinant plasmid pTHI9 was transformed into E.coli BAL 31 and induced by addition of 1 mmol/L IPTG into the culture at logarithmic phase. Analysis by SDS-PAGE and Western-blot revealed that the insecticidal peptide was produced by inducement. The product made up 13. 75% of the total bacteria proteins and proved to be active via assay of insect toxicity.
基金
澳大利亚Deakin公司
北京大学蛋白质工程及植物基因工程国家重点实验室资助
关键词
蜘蛛杀虫肽
基因表达
活性
Spider insecticidal peptide, Gene expression, Insect toxicity
