摘要
采用ＨＰＩＥＣ应用ＣＭ交换柱分离纯化Ｅ．ｃｏｌｉ工程菌表达的ｒＩＬ－２，与空气氧化复性相比较，又有１倍以上的变性ｒＩＬ－２在色谱过程中得到复性，且溶液ｐＨ影响ｒＩＬ－２的复性和纯化效果：ｐＨ７．０时ｒＩＬ－２复性率高于ｐＨ８．０时，但纯度明显低于ｐＨ８．０时分离的ｒＩＬ－２．
By using high pressure ion exchange chromatography (HPIEC) of CM exchanger to purify recombinant interleukin-2 (rIL-2) expressed by engineered E. coli,about twice more the amount of rIL-2 were renaturated during the chromatographic purification. The renaturation was affected by PH of the buffer,the renaturation rate of rIL-2 in PH7.0 buffer was higher than that in PH8.0,but the purity was lower than that in PH8.0.
关键词
白细胞介素-2
色谱法
制备
Interleukin-2, Chromatography, Protein purification
