GPX－abzyme对受XO／HX系统损伤的心肌线粒体的保护作用

Protection of Glutathione Peroxidase-abzyme on Myocardial Mitochondria in XO/HX Injuring System

探讨了研制的具有谷胱甘肽过氧化物酶活力的含硒抗体酶（ＧＰＸ－ａｂｚｙｍｅ）对于受损心肌线粒体的保护作用，利用牛的心肌线粒体为实验材料，通过线粒体的膨胀度、脂质过氧化物含量、ＣＣＯ活力变化及电镜观察等几个方面证明ＧＰＸ－ａｂｚｙｍｅ能抵抗ＸＯ／ＨＸ系统产生的自由基的损伤作用，ＥＳＲ研究也表明ＧＰＸ－ａｂｚｙｍｅ能明显降低ＸＯ／ＨＸ损伤系统中的自由基含量。

GPX-abzyme was prepared and its protective function to the injured myocardial mitrochondia was investigated. Using the bovine myocardial mitochondria as experimental material,the results showed that the GPX-abzyme may inhibit free radical injuries by the XO/HX system studying the swelling of mitochondia,quantity of lipid peroxide production,changes of CCO activity and the transmission electron microscopic observation.Studies of the ESR changes indicated that GPX-abzyme could obviously decrease the amount of free radical in the XO/HX injuring system.