摘要
采用垂直板聚丙烯酰胺凝胶电泳和紫外分光光度法分析大鲵１５种组织的乳酸脱氢酶（ＬＤＨ）同工酶后发现：在正常生理条件下，大鲵体内只有Ｈ４和Ｍ４两种同工酶，红细胞只有Ｍ４一种同工酶；大鲵体内ＬＤＨ同工酶中，以Ｍ４占绝对优势；除生殖腺及脑外，在幼体至成体的发育过程中，ＬＤＨ同工酶未见变化；大鲵体内各组织ＬＤＨ含量及前述两种同工酶相对含量不同。
Under normal conditions,two kinds of lactate dehydrogenase(LDH) isozylnes H4 andM4 were found in Megalobatrachus davideinus. The other three kinds of isozymes(H1M3,H2M2,H3M1)were not fond.The results of the LDH isozymes from fifteen tissues showedthat each of the fourteen tissues contains two kinds of isozymes except the red cell.The redcells have only one(M4).But the relative content in each tissue was different.M4 isozymewas absolutely predominant compared with the other isozymes. During the development process from young to adult,the contents of LDH isozymes in tissues of M.davidianus were notchanged except the brain and spermary.The content of LDH in every investigated tissues aredifferent from each another.
出处
《水生生物学报》
CAS
CSCD
北大核心
1996年第3期265-270,共6页
Acta Hydrobiologica Sinica
关键词
大鲵
乳酸脱氢酶
同功酶
组织分布
Megalobatrachus davidianus
LDH isozyme
Tissues distribution
