雌雄蚕蛹近红外反射光谱的差异及其模式识别

DIFFERENCE BETWEEN MALE AND FEMALE PUPAE OF BOMBYX MORI IN NEAR INFRARED REFLECTANCE SPECTRA AND THEIR PATTERN RECOGNITION

采用６２５０型近红外光谱分析仪，从波长６８０到１２３５ｎｍ分别对日系蚕蛹２１７颗、中系蚕蛹１７４颗、杂交种蚕蛹３３０颗做了扫描测试，结果表明：雌雄蚕蛹的近红外光谱有极显著的差异。从日系２１７颗蚕蛹中随机选取雌雄各５０颗作为学习样本，采用逐步判别方法从其二阶导数光谱中抽取５个特征波长作为观测向量，利用二次型判别函数对其余６２１颗蚕蛹的性别进行识别，其准确率达９８．７％。该方法明显优于以蚕蛹重量或大小识别蚕蛹雌雄的方法。

In this paper, difference between male and female pupae of Bombyx mori in near infrared reflectance spectra(NIRS) and the feasibility and method of spectra recognition to identify the sex of fresh pupae were studied. By Model 6250 NIR instrument, indestructive determination of 721 fresh silkWorm pupae selected from Chinese race, Japanese race and their hybrid was made with suitable wavelengths chosen between 680urn and 1235nm. Fifty male and fifty female pupae was randomly selected from 217 pupae of Japanese race as learning samples. The feature at 5 wavelengths was extracted as observation vector from data of their second derivative spectra by a stepwise discriminant analysis. Statistical analysis showed that there were extremely significant differences between male and female pupae in NIRS at the 5 wavelengths. When the Bayes quadratic discriminant function built by the feature was used to predict the sex of the other 621 test samples, 98.7% of correct rate of the identification recognition was obtained. ms method is much better than other identification ones which use weight or size of pupae as their feature. The mechanism is discussed.