非繁殖季节太行山羊胚胎移植方案研究

Studies on the scheme making embryo transfer in taihang goats at non-breeding season

对48只太行山羊(供体羊)、40只太行山羊和120只奶山羊(受体羊)进行了同期发情和超数排卵胚胎移植试验。其结果如下:(1)CIDR+PMSG对太行山羊同期发情,有效发情率为75%;CIDR+FSH对奶山羊同期发情,有效发情率为73%。(2)CIDR+FSH+LH组平均可用胚胎数分别与CIDR+PMSG组和CIDR+PMSG+LH组之间差异达到极显著水平(P<0.01),而与CI-DR+FSH组之间差异显著(P<0.05)。(3)CIDR+FSH+LH超排时,用CIDR+FSH对奶山羊进行同期发情,受体妊娠率为56%;CIDR+PMSG对太行山羊同期发情,受体妊娠率为60%水平。证明在非繁殖季节对太行山羊采用CIDR+FSH+LH超数排卵和以太行山羊及奶山羊为受体分别用CIDR+PMSG和CIDR+FSH同期发情来进行胚胎移植的技术方案是可行的。

Superovulation was conducted in 48 Taihang Goats and synchronized estrus was induced by associated hormones and CIDR methods in 40 Taihang Goats and 120 Milk Goats. The results showed： It was applied synchronized estrus in Taihang goats and milk goats to the CIDR＋ PMSG and CIDR＋FSH groups respectively, which the percentage of effective estrus is 75% and 73% respectively. （2） There were respectively significant difference of average usable embryos between CIDR ＋FSH＋LH group to CIDR＋PMSG and CIDR＋PMSG＋LH groups （P〈0.01）, there was difference of average usable embryos between CIDR＋FSH＋LH group to CIDR＋FSH group（P〈0.05）. （3） When superovulation was conducted by CIDR＋ FSH ＋ LH group in Taihang Goat, CIDR＋ FSH group and CIDR＋ PMSG group were applied synchronized estrus in milk goats and Taihang goats which rate of pregnancy are 56% and 60% respectively. Wherefore, it is feasible to scheme of embryo transfer which superovulation was conducted in Taihang Goats and Synchronized estrus was induced in Taihang Goats and milk goats at non-breeding season.