大黄提取片对高脂饮食诱导肥胖大鼠脂代谢及相关基因表达的影响

Effects of Refined Rhubarb on Lipid Metabolism and Related Genes Expression in High Fat Diet Induced Obese Rat

目的:观察大黄提取片对高脂饮食诱导肥胖大鼠脂代谢、骨骼肌脂联素受体1(AdipoR1)和过氧化物酶体增殖物激活受体a(PPARa)基因表达的影响,以探讨大黄的减肥作用机理。方法:给饮食诱导肥胖大鼠灌服低、中、高剂量(200,400,800mg.kg-1.d-1)大黄提取片,测定血脂、脂联素,应用Real-time PCR检测大鼠骨骼肌中AdipoR1和PPARa mRNA表达水平。结果:大黄提取片呈剂量依赖性的增强降脂作用,高剂量大黄提取片能显著降低肥胖大鼠血总胆固醇(CHO)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA),显著增加骨骼肌组织PPARa mRNA表达水平,但3种剂量大黄均不能明显改变脂联素和骨骼肌AdipoR1mRNA水平。结论:大黄提取片可通过增加肥胖大鼠骨骼肌PPARa表达、降低血脂水平而促进机体减肥。

Objective： To investigate the effects of Refined Rhubarb on hpid metabohsm and genes expression of adiponectin receptorl （ AdipoR1 ） and peroxisome proliferator-activated receptor a （PPARa） in skeletal muscle of high fat diet induced obese rat, and further explore anti-obesity mechanism of Rhubarb. Methods： High fat diet induced obese rats were administrated with different dosage （200, 400, 800mg·kg^-1·d^-1） for two months. Lipid metabolic parameters and serum adiponectin were measured; the expression levels of AdipoR1 and PPARa mRNA were determined by Real-time PCR. Results： Refined Rhubarb dose-dependently lowered lipid level. High dose of Refined Rhubarb treatment decreased serum total cholesterol （CHO）, triglyceride （TG）, low density lipoprotein cholesterol （LDL-C）, free fat acid （FFA） and increased expression of PPARa mRNA in skeletal muscle significantly, while serum adiponectin and AdipoR1 mRNA in skeletal muscle were not altered. Conclusion： Refined Rhubarb has significant anti-obesity effect via up-regulating PPARa expression and reduced lipid parameters.