参麦注射液对“双击”大鼠肝脏IκBmRNA表达的影响

Influence of Shenmai injection on the expression of IκBmRNA in the "two-hits" rats

目的:探讨参麦注射液的抗休克作用及其机制。方法:大鼠随机分为实验对照(Control)组、双击(HS+LPS)组、参麦注射液小剂量(HS+LPS+SML)组和参麦注射液大剂量(HS+LPS+SMH)组。大鼠动脉放血至平均动脉压40mmHg,维持10min。然后舌下静脉注射内毒素(1.5mg·kg-1),4h后检测血清NOS活性、NO含量和肝脏IκBmRNA的表达。结果:HS+LPS+SML组和HS+LPS+SMH组血清NO含量、NOS活性明显低于HS+LPS组(P<0.05),而IκBmRNA表达明显高于HS+LPS组(P<0.05)。结论:参麦注射液通过上调IκBmRNA的表达,降低NO含量和NOS活性,对组织细胞具有保护作用。

Objectlve To explore the anti-shock effect and molecular mechanism of Shenmai injection. Method Rats were divided into （HS＋LPS） group, （HS＋LPS＋SML） group, （HS＋LPS＋SMH） group and the control group randomly, Serumal NOS activity, NO content and liver tissue＇s IkBmRNA expression were assayed 4 hours after ＂two-hits＂. Results NOS activity and NO content in （HS＋LPS＋SML） group and （HS＋LPS＋SMH） group were significantly lower than that in. （HS＋LPS） group （P〈0.05）, while IkBmKNA expressions in the liver tissue were markedly increased compared withthat of （HS＋LPS） group. Conclusion Shenmai injection may through up-regulating IkBmKNA expression in the liver tissue, set down the NOS activity and NO content induced by ＂two-hits＂ and provide protective effects on histiocyte.