降解2-氯酚厌氧污泥中微生物种群结构研究

Study on Microbial Community Structure in Anaerobic Granular Sludge for 2-chlorophenol-degradation

采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术,研究Fe2+,Ni2+金属离子对经2-氯酚(2-CP)驯化后的厌氧生物反应器颗粒污泥的微生物种群结构的影响.结果表明,经2-CP驯化后,厌氧体系微生物多样性减少,产生了能适应或降解2-CP的主要菌群发光杆菌属、埃希氏菌属和志贺氏菌属.投加Fe2+,Ni2+后,降解2-CP的厌氧污泥微生物多样性均呈减少趋势,其中,加Fe2+后的厌氧体系微生物多样性明显减少,并产生新的条带,经分析,证明为专性厌氧梭菌属.

The influence of Fe^2＋ and Ni^2＋ metal ions on microbial community structure in 2-chlorophenol （2-CP）-acclimated anaerobic granular sludge was investigated with modern molecular biological techniques. Total DNA was extracted directly from anaerobic granular sludge and was amplified with polymerase chain reaction （PCR） technique. The PCR products were fingerprinted by denaturing gradient gel electrophoresis （DGGE）. Biodiversity of microbial community decreased in 2-CP-acclimated anaerobic granular sludge and some predominant microbes which can adapt to or degrade 2-CP were developed after acclimation, including Photorhabdus luminescen genus, Escherichia genus and Shigella genus. After adding Fe^2＋ and Ni^2＋ to the anaerobic system, biodiversity of microbial community decreased dramatically and a new band was observed in Fe^2＋-added sludge, which was verified to be Clostridium genus.