4种淡水藻对硝基苯的抗性机制

Resistant mechanism against nitrobenzene in four species of freshwater algae

选择了4种淡水藻———纤细裸藻、莱因衣藻、舟形藻和聚球藻,通过分析硝基苯对其生长和叶绿素含量的影响,以及藻细胞对硝基苯的降解、积累和抗氧化酶系统的响应,以期了解藻类对硝基苯的抗性机制.结果表明:4种藻的生长和叶绿素a含量对硝基苯的抗性,按抗性大小排列:纤细裸藻>莱因衣藻>舟形藻>聚球藻,4种藻对硝基苯无显著的降解和积累能力,藻细胞对硝基苯的抗性主要依赖抗氧化酶系的防御作用,其中藻细胞的SOD起主要作用,POD起次要作用.

Four species of typical freshwater algae--Euglena gracilis, Chlamydomonas reinhardtii, Navicula pelliculosa and Synechococcus leopoliensis were selected as the experiment materials. Effects of nitrobenzene on the growth and chlorophyll a content of the algae, the biodegradation and bioaccumulation of nitrobenzene, and the response of antioxidant enzymes to nitrobenzene were investigated, in order to reveal the resistant mechanism against nitrobenzene in the algae. The results indicated that the growth and chlorophyll a in the four sepecies of algae showed similar response to nitrobenzene, the order of resistance were E. gracilis〉 C. reinhardtii 〉 N. pelliculosa 〉 S. leopoliensis. No significant biodegradation and bioaccumulation of nitrobenzene were found in the four species of the algae. The resistance of the algae against nitrobenzene mainly rely on the antioxidant system. SOD played the most important role and POD also showed functions in the resistence mechanism in the algae.