恶性血液病患者化疗前后淋巴细胞亚群测定及其意义

Detection of lymphocyte subsets before and after chemotherapy in hematologic malignances and its clinical significance

目的研究恶性血液病患者化疗前后淋巴细胞亚群的变化及其意义。方法采用流式细胞术对21例急性淋巴细胞白血病(ALL)/非霍奇金淋巴瘤(NHL)患者和23例急性髓系白血病(AML)患者化疗前后淋巴细胞亚群(包括CD+4,CD+8,CD+4/CD+8比值,NK细胞)进行检测。结果CR组的CD+4/CD+8比值与NK细胞比例均明显高于初诊组和化疗后未缓解组(P<0.05);CR组ALL/NHL的CD+4/CD+8比值明显低于AML(P<0.05)。结论CD+4/CD+8比值与NK细胞比例可以作为判断恶性血液病患者病情严重程度和预后的指标,与AML相比,ALL/NHL的细胞免疫功能受损更为严重。

Objective To study the changes of lymphocyte subsets before and after chemotherapy in hematologic malignances and it＇s clinical significance. Methods The different level of lymphocyte subsets of 21 ALL/NHL patients and 23 AML patients were detected before and after chemotherapy with FCM. Results The levels of CD4^＋ / CD8^＋; and NK cells of CR group leukemia/lymphoma were. significantly higher than that of leukemia/lymphoma of NR and newly diagnosed group（P 〈0.05）; The levels of CD4^＋/CD8^＋ and NK cells of CR group ALL/NHL were significantly lower than that of CR group AML （P〈0.05）. Conclusion The levels of CD4^＋ / CD8^＋; and NK cells detection are helpful in predicting prognosis and ponderance of hematologic malignances. Compared to AML, the immunity damage of ALL/NHL was more severe.