碱性成纤维细胞因子对真皮下血管网皮瓣成活影响的实验研究

BFGF improves the viability of subdermal vascular network flap

目的研究碱性成纤维细胞生长因子(bFGF)对促进随意型真皮下血管网皮瓣(SVNF)成活的作用。方法分别在4只纯白色家猪两侧背部各形成6个SVNF,长16cm,宽4cm,将24个皮瓣随机分为bFGF组和对照组,于术后6d测量各组皮瓣成活率及皮瓣新生血管密度。结果术后6d,bFGF组与对照组SVNF成活率分别为(81.2±1.7)%和(66.2±1.8)%(P<0.05),皮瓣近、中、远段皮瓣血管密度分别为(16.7±6.0)、(29.7±5.5)、(14.9±6.0)/mm2和(16.6±4.0)、(21.4±5.2)、(3.5±5.1)/mm2(P<0.05)。大体观察和组织学观察bFGF组均优于对照组。结论皮瓣下局部注射bFGF后,通过促进SVNF远端基底部和边缘部的血运,可以提高SVNF成活率,缩短皮瓣的断蒂时间,扩大皮瓣长宽比例。

Objective ：TO Investigate the application valve of DFGF to Improve the viability of subdermal vascular network flap. Methods ：Four white healthy pigs were used,each pig had 6 on back,16cmx 4cm,totally 24 flaps were divided randomly into 2 groups：The DFGF groups and the control groups. On postoperative day 6, we observed the appearance of the flaps. The survival area of each flap was measured and the flap was harvested for histological analysis and the density of anglogenesis was also examined, Results ： On Postoperative day 6, the survival rates of the bFGF group and the oontrol group were （81.2 ± 1.7）% and （66.2 4±1.8）% respectively,the angiogenesls denslty of the middle and the distal segment in the bFGF group was （16. 7 ±6.0） /mm^2, （29.7± 5.5） /mm^2, （ 14.9 ± 6. 0）/mm^2 respectively, while （16.6 4± 4. 0） /mm^2, （21.4 ± 5.2）/mm^2 , （3.5 ±5.1）/mm^2 respectively in the control group. The bFGF group was superior to the control group in the appearance and histological analysis of the flaps. Conclusion ：The results suggest that the injection of bFGF locally can promote the survival rate by accelerating the blood circulation of the SVNF＇ bottom and edge, shorten the division time of SVNF and broaden the ratio of length and width.