ET-A在脑血管畸形血管中表达的实验研究

Expression of ET-A mRNA in cerebral vascular malformation

目的探讨内皮因子受体A(ET-A)mRNA在脑血管畸形血管中的差异性表达。方法对14例脑血管畸形和8例正常脑血管标本分别提取总RNA,应用逆转录-聚合酶链反应的方法检测ET-AmRNA的表达,然后行DNA电泳鉴定,并进行光密度扫描,半定量计算ET-AmRNA的表达。结果在脑血管畸形血管中ET-AmRNA表达水平较正常脑血管中明显下降(P<0.05)。结论脑血管畸形血管与正常脑血管中ET-AmRNA表达有差异性,为研究其在病理情况下的作用作了前期工作。

Objective To explore the change of endothelin-A （ET-A） mRNA expression in cerebral vessels of the patients with cerebral vascular malformation. Methods Total RNA was extracted from 14 samples of cerebral vascular malformation, which were removed by microneurosurgery, and from 8 samples of normal cerebral vessels. Expression of ET-A mRNA in cerebral vessels was detected by RT-PCR, DNA electropherosis, photodensity scan. Results The expression of ET-A mRNA in cerebral vascular malformation was lower than that in normal cerebral vessels （P〈0.05）. Conelusion The expression of ET-A mRNA is different between in normal cerebral vessels and in cerebral vascular malformation, which offers a basis for studying the pathogenesis of cerebral vascular malformation.