摘要
几研究证明了根瘤细菌属 nifA 基因是氮固定在小瘤细菌行动的使活跃之物。在苜蓿上理解 Sinorhizobium meliloti nifA 基因的效果， cDNA-AFLP 技术被采用在 nifA 异种小瘤在基因表示学习变化。在约 3,000 导出抄本的碎片之中， 37 有微分表示层次。这些表示层次被反向的北污点和 RT 聚合酶链反应随后证实。顺序分析表明 21 cDNA 碎片对应于涉及信号通讯，蛋白质降级，滋养的新陈代谢，房间生长和开发的基因。
Several studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in nodule bacteria. To understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa, the cDNA-AFLP technique was employed to study the changes in gene expression in nifA mutant nodules. Among the approximately 3,000 transcriptderived fragments, 37 had differential expression levels. These expression levels were subsequently confirmed by reverse Northern blot and RT-polymerase chain reaction. Sequence analyses revealed that 21 cDNA fragments corresponded to genes involved in signal communication, protein degradation, nutrient metabolism, cell growth and development.
