PIKA佐剂在体内外诱导小鼠免疫应答的探讨

PIKA adjuvant induces the immune responses in mouse in vitro and in vivo

目的探讨PIKA佐剂在体内外诱导小鼠的免疫应答。方法体外将小鼠脾淋巴细胞与不同浓度PIKA佐剂培养后,ELISA检测细胞因子IFN-γ、IL-12p40的产生,FACS检测细胞的增殖及活化。体内将PIKA佐剂经小鼠腹腔注射后,检测不同时间点血清中IFN-γ、IL-12p40、IL-6、TNF-α等细胞因子的产生。结果PIKA佐剂体外呈剂量依赖性诱导小鼠脾细胞产生IFN-γ和IL-12p40。细胞亚群分析的结果表明,PIKA可显著刺激B细胞和NK细胞活化、增殖。体内注射PIKA佐剂后可诱导细胞因子IFN-γ、IL-12p40、IL-6、TNF-α的产生,但产生的时间点不同。结论PIKA佐剂体内外直接通过诱导细胞因子的产生,B细胞和NK细胞的活化和增殖,而促进免疫应答反应。

Objective：To evaluate immune responses of mouse induced by PIKA adjuvant both in vitro and in vivo. Methods： Mouse splenocytes were prepared and cultured with different concentrations of PIKA adjuvant. The production of IFN-γ and IL-12p40 was determined by ELISA,and cell activation and proliferation was evaluated in vitro by FACS. For in vivo studies, mouse was injected （i. p. ） with PIKA adjuvant and cytokines such as IFN-γ,IL-12p40, IL-6,TNF-α in serum were assayed by ELISA at different：time points after injection. Results： PIKA adjuvant induced production of IFN-γ and IL-12p40 by mouse splenocytes in a dose-dependent manner in vitro. Cellular analysis demonstrated that PIKA elicited the activation and proliferation of B ceils and NK cells but not CD4^＋ and CD8^＋ T ceils. Following injection, PIKA adjuvant induced the production of IFN-γ, IL-12p40, IL-6, TNF-α in vivo With different kinetics. Conclusion：PIKA adjuvant may directly enhance immune responses via the induction of cytokine secretion, activation and proliferation of B and NK ceils.