雷公藤多苷对内毒素激活小鼠腹腔巨噬细胞分泌促炎症细胞因子的影响

Effect of polyglycoside of trip terygium wilfordii hook.f. on inflammatory cellular factors of peritoneal elicited macrophage activated by lipopolysaccharide in mice

目的观察雷公藤多苷(TWP)对细菌内毒素(LPS)激活小鼠腹腔巨噬细胞(Mφ)分泌促炎症性细胞因子TNF-α和IL-6的影响,以探讨过度炎症反应中高细胞因子血症的调控药物和措施。方法分离纯化小鼠腹腔Mφ,用LPS激活,与TWP共同孵育;以间接MTT法和ELISA法检测培养上清液中的TNF-α和IL-6的浓度。结果TWP在浓度6.25～100μg/L,时间4～24小时范围内,对Mφ产生TNF-α具有显著抑制作用(P<0.01),呈剂量和时间依赖关系;TWP在浓度6.25～50μg/L范围内,时间12小时,对Mφ产生IL-6具有显著抑制作用(P<0.01),呈剂量依赖关系。结论TWP可以抑制LPS激活Mφ分泌促炎症性细胞因子的活性。

Objective：To study the effect of polyglycoside of trip terygium wilfordii hook. f. on inflammatory cellular factors of peritoneal elicited macrophage activated by lipopolysaccharide in mice, to investigate control drug or measure in hyperecytokinemia. Methods：The peritoneal elicited macrophage were separated, purified and activated by LPS in mice, then cultured in vitro with a series concentration of TWP, at the same time the macrophages were activated by LPS. The TNF-α activity and IL--6 level of cellular supernatants were determined by MTT colorimetric assay and ELISA respectively. Results：The activity of TNF-α and the level of IL-6 in Mφ, could be significantly inhibited（ P 〈0. 01 ） by TWP（6. 25-100 μg/L and 6. 25-50 μg/L, respectively） during 4-24 h and 12 h respectively in a time and dosage dependent manner. Conclusion：TWP can inhibit the TNF-α activity and IL-6 level on macrophage activated by LPS.