2004～2005年冬春季急性下呼吸道感染患儿RSV亚型的检测及分析

Characterization of respiratory syncytial virus subgroup A and B in infants with acute lower respiratory tract infection from winter 2004 to spring 2005

目的分析2004～2005年冬春季呼吸道合胞病毒(RSV)流行季节的亚型分布情况。方法采用直接免疫荧光法检测2004年10月～2005年4月间因急性下呼吸道感染住院儿童1040例鼻咽部分泌物中的常见病毒病原,随机选择RSV阳性标本174例,采用间接免疫荧光检测RSVA、B亚型,分析检测结果。结果1040例中,RSV阳性497例(47.8%),其中174例标本中,检测出RSVA亚型23例(13.2%),B亚型105型(60.4%),A、B亚型混合感染36例(20.7%),无法确定亚型的10例(5.7%)。检测期间各月份间、不同的年龄组间和临床诊断间的RSV亚型分布差异均无显著性意义(P>0.05),都以B亚型为主。结论2004～2005年冬春季RSV仍是儿童急性下呼吸道感染的主要病原,以B亚型为主;并有两种亚型同时感染的情况。

Objectives To investigate epidemiological features of respiratory syncytial virus （RSV） A and B in infants with acute lower respiratory tract infections from winter 2004 to spring 2005. Methods Nasopharyngeal secretions were sampled from 1 040 hospitalized infants with acute lower respiratory tract infection and were tested with direct immunofluorescence assay for seven common respiratory viral antigens including RSV. Indirect immunofluorescence assay was used to test for RSV subgroup A and B in 174 samples randomly selected from 497 with detected RSV. Results Among 174 samples RSV subtype B were detected in 105 （60.3 %）, RSV subtype A in 23 （13.2 %）, both RSV subtype A and B in 36 （20.7 %） and RSV subtype of the other 10 （5.7 %） were unclear. No significant differences in the prevalence of RSV subgroups were found among months, ages or clinical presentations （P 〉 0.05 for aiD. Conclusions RSV subtype B was the primary pathogen in children with lower respiratory tract infection in Wenzhou from winter 2004 to spring 2005 and combined infection of RSV A and B were also identified,