运动病大鼠血浆降钙素基因相关肽含量的变化

Effect of rotatory stimulation on plasma level of calcitonin generelated peptide in rats

目的:探讨降钙素基因相关肽(CGRP)在运动病发病过程中的作用.方法:第1组30只大鼠按随机数字法分为4个实验组和1个对照组,实验组动物给予30 m in旋转运动刺激,然后各自测4个实验组动物旋转运动刺激后20 m in,6h,1 d,3 d以及对照组动物血浆CGRP含量;第2组20只大鼠先给予15 m in的旋转运动刺激,根据刺激前后24 h糖精水饮用量的变化分为运动病敏感组和不敏感组,然后测两组动物旋转运动刺激15 min后血浆CGRP含量.结果:第1组动物,实验组动物旋转运动刺激30 min后各时间点大鼠血浆CGRP含量和对照组差异没有显著性意义(F=1.105,P=0.893);第2组动物,运动病敏感组与运动病不敏感组相比,旋转运动刺激15 min后血浆CGRP含量差异无显著性意义(P=0.704).结论:血浆CGRP在大鼠运动病发病机制中没有发挥作用;大脑中枢和前庭终器中CGRP是否参与了运动病的发病尚需深入研究.

AIM： To explore the relation between calcitonin gene-related peptide （CGRP） and motion sickness（MS） in rats. METHODS： A group of 30 rats were subdivided into 4 experimental groups and a control group with a random number table. The rats of experimental groups were treated with rotatory stimulation for 30 min. Then the plasma levels of CGRP in 4 experimental groups were measured respectively 20 min, 6 h, 1 and 3 d after the stimulation. According to the changes of rat＇s intake of saccharin solution before and 24 h after rotatory stimulation for 15 min. Another group of 20 rats were subdivided into a group sensitive to MS and a group insensitive to MS, then the plasma levels of CGRP in the 20 rats were measured 20 min after rotatory stimulation for 15 min. RESULTS： There was no significant difference in the plasma levels of CGRP at different time points after rotatory stimulation in the first group （ F = 1. 105, P = 0. 893 ） ; there was no significant difference in the plasma levels of CGRP between the group sensitive to MS and the group insensitive to MS （P = 0. 704）. CONCLUSION： The CGRP in blood plasma doesn＇t play an role in the pathogenesis of MS.