PCNA和MMP9在宫颈癌组织中的表达与意义

Expressions and significance of PCNA and MMP9 in cervix cancer tissue

目的:探讨PCNA,MMP9在宫颈癌组织中的表达及临床意义.方法:用免疫组织化学SP法检测46例宫颈癌及10例正常宫颈中PCNA和MMP9的表达,分析不同组织中PCNA和MMP9表达的差异及两者的相关性.结果:宫颈癌组织PCNA的表达明显高于正常宫颈组织(P=0.000);38例宫颈癌的MMP9阳性表达(82.61%)明显高于10例正常宫颈组织的表达(30%,P<0.05);PCNA的表达与MMP9的表达呈正相关(rs=0.433,P=0.003),且PCNA和MMP9均与临床分期有关(P<0.05),而与年龄、病例分级及生长方式无关(P>0.05).结论:MMP9在宫颈癌组织中表达异常增高,与细胞增殖关系密切,提示其对宫颈癌的发展起重要作用.

AIM： To investigate the expressions of PCNA and MMP9 in cervix cancer tissue and their significance. METHODS： The immunohistochemical SP staining method was used to evaluate the PCNA and MMP9 expressions in 46 cases of cervix cancer tissues and 10 cases of normal cervix tissues. RESULTS： The expression of PCNA in cervix cancer tissues was much higher than that in normal cervix tissues （ P = 0.000 ） ; The positive rate of MMP9 protein 82.61% in 46 cases of cervix cancer was significantly higher than that in 10 cases of normal cervix （30%, P 〈 0. 05 ）. There was a positive correlation between PCNA and MMP9 expressions （ re = 0. 433, P = 0. 003 ）. The expressions of PCNA and MMP9 were correlated with the clinical stage （ P 〈 0.05 ）, but not with age, pathological stage or growth pattern （ P 〉 0.05 ）. CONCLUSION ： The over-expression of MMP9 has a close relationship with the cell proliferation index in cervix cancer, suggesting that MMP9 may play an important role in the development of cervix cancer.