二硫化碳对大鼠支持细胞凋亡及FasL表达的影响

Apoptosis and gene FasL expression induced by carbon disuifide in rat steroli cells

目的探讨二硫化碳对离体培养支持细胞凋亡及FasL基因表达的影响。方法以0、0．36、0．72、1．44μmol/ml浓度的二硫化碳作用于离体培养的大鼠支持细胞24 h后,应用噻唑蓝(MTT)法检测支持细胞存活率,流式细胞术检测细胞凋亡及逆转录聚合酶链反应(RT-PCR)技术检测凋亡相关基因FasL的表达。结果二硫化碳处理后的支持细胞存活率随作用浓度的增加而下降,当≥1．44μmol/ml浓度时,细胞存活率(73．34%±1．39%)明显下降,与溶剂对照组(99．98%±5．48%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度升高,细胞凋亡率增加,当浓度≥1．44μmol/ml时,支持细胞凋亡率(7．93%±0．43%)明显升高,与溶剂对照组(3．63%±0．53%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度增加,凋亡相关基因FasL表达量亦明显增加。结论二硫化碳对离体培养支持细胞具有细胞毒性作用,可导致细胞凋亡率升高,凋亡相关基因FasL高表达。

Objective To study apoptosis and gene FasL expression induced by carbon disulfide in steroh cells of male rats. Methods Steroli cells were exposed to different concentrations of CS2（0,0.36,0.72,1.44 μmol/ml） for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively. Results Steroli cell survival rate decreased as the concentration of CS2 increased. The survival rate（73.34% ± 1.39%） was significantly lower than the control group（99.98% ± 5.48% ） when the concentration of CS2≥ 1.44 μmol/ml（ P 〈 0.05）.Apoptosis rate increased as the CS2 concentration increased. Apoptosis rate（7.93 % ± 0.43 % ） was significantly higher when the concentration of CS2 ≥ 1.44 μmol/ml （ P 〈 0.05）. Expression level of the FasL significantly increased as the concentrations of CS2 （ P 〈 0.05）. Conclusion CS2 is cytotoxic to steroli cells. It could cause apoptosis of steroli cells.