高功率微波辐射后下丘脑神经元凋亡和线粒体膜电位与Ca^(2+)的变化

Changes of apoptosis,mitochondrion membrane potential and Ca^(2+) of hypothalamic neurons induced by high power microwave

目的探讨高功率微波辐射后下丘脑神经元凋亡、线粒体膜电位与Ca^(2+)的变化。方法以10与30 mW/cm^2高功率微波辐射原代培养的下丘脑神经元,于辐射后6 h采用倒置显微镜和流式细胞术检测细胞的凋亡、线粒体膜电位与胞浆钙离子的变化。结果辐射后6 h,10与30 mW/cm^2组凋亡率与假辐射组相比均明显增加；30 mW/cm^2组辐射后坏死率与假辐射组比较亦明显升高,差异有统计学意义(P＜0．05)。辐射后6 h,30 mW/cm^2组Ca^(2+)明显升高,膜电位性明显下降,差异均有统计学意义(P＜0．01)。结论细胞凋亡是下丘脑神经元死亡的主要方式之一,胞浆内Ca^(2+)超载及线粒体膜电位的下降参与其损伤过程。

Objective To explore the injury effect and mechanism of hypothalamic neurons after high power microwave（HPM） exposure. Methods Primarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm^2 HPM, and the inverted phase contrast microscope（IPCM） and flow cytometry（FCM） were employed to detect the injury of cells and change of mitochondrion membrane potential（MMP） and Ca^2＋ in the cytoplasm of neurons. Results The ratio of apoptosis was significantly higher than that of the sham exposure（ P 〈0.05） induced by 10 and 30 mW/cm^2 HPM and necrosis increased significantly（ P 〈0.05） in the group of 30 mW/cm^2 at 6 h after exposure. The content of Ca^2＋ in the cytoplasm of neuron cells increased（ P 〈 0.01 ） while MMP decreasedsignificantly（P〈0.01） after radiation of 30 mW/cm^2 HPM at 6 h after exposure. Condusion Apoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca^2＋ and the decline of MMP axe involved in the process.