高效液相色谱法测定清热化毒丸中牛蒡苷的含量

Determination of Arctiin in Qingrehuadu Pill by HPLC

目的：建立清热化毒丸中牛蒡苷的含量测定方法。方法：采用HPLC法，以十八烷基硅烷键合硅胶为填充剂；以甲醉-乙腈-0．025mol／L的磷酸（29：23：48）为流动相；检测波长为280nm。结果：牛蒡苷进样量在0．794—3.968μg之间呈良好的线性关系（r=0．9995），平均加样回收率为95．2％，（RSD=1.4％，n=5）。结论：本法灵敏、准确、重现性好，精密度高、专属性强。可用于清热化毒丸的质量控制。

Objective： To establish the detetmination of Arctiin in Qingrehuadu Pill. Methods：The determination was carried out by HPLC witb octodelyl silicohydrides silanes bonded silica gel as a packing material, Methanol - Acetonitrile - 0. 025mol/L phosphoric （29： 23： 48）as a mobile phase,detection wavelength at 280nm. Results： Arctiin showed a good linear relationship at the range of 0.794 -3. 968μg （ r = 0. 9995 ） ; The average recovery of Arctiin was 95.2% （ RSD = 1.4%, n = 5 ）, in the tablets. Conclusion ： The method issensitive, accurate, reproducible, specific and can be used for quality control of Qingrehuadu Pill.