金雀异黄素和5-FU对人结肠癌细胞株(Colo320)的相互作用的体外观察

The combined effect of Genistein and 5-FU on human colorectal cancer cell lineColo-320

目的体外观察金雀异黄素(Gen iste in)和5-氟脲嘧啶(5-FU)合用对人结肠癌细胞株(Colo320)的相互作用。方法采用MTT法利用中效原理判定2药合用的效果。结果2种药物单独应用时随着药物剂量的增加其效应增加,合用时2药大剂量时(>中效浓度)具有拮抗作用,小剂量(<中效浓度)时具有协同作用;2药给药时间及给药顺序都会影响效应的大小。结论上述2种药合用时大剂量时有拮抗作用,小剂量时有协同作用且效应大小与给药时间及给药顺序有关。

Objective To observe effects of Genistein and 5-fluorouracil （5-FU） on human colon carcinoma cell line Colo320. Methods The MTr assay and median-effect principle were used. Results The two drugs were antagonistic at higher concentrations and synergistic at lower concentrations, The sequence and time of drug administration can influence the effects of the two drugs on Colo320. Conclusion The two drugs were cooperated at lower concentrations and antagonistic at higher concentrations. The sequence and time of drug administration were also important for their effects on the cells.