血管内皮生长因子在大肠癌组织中的表达及其与骨髓微转移的关系

Expression of vascular endothelial growth factor in colorectal carcinoma and its correlation with tumor bone marrow micrometastasis

目的研究血管内皮生长因子(VEGF)在大肠癌组织中的表达,探讨其与骨髓微转移的关系。方法应用免疫组织化学法检测70例大肠癌组织标本中VEGF的表达,采用膜表面抗原(EMA)单克隆抗体检测其患者术前骨髓中转移癌细胞。结果VEGF在52.9%(37/70)的大肠癌患者中呈阳性表达;VEGF在有骨髓微转移组中的表达与无骨髓微转移组比较,差异有统计学意义(P<0.01)。VEGF的表达和骨髓微转移联合检测与远处转移的关系更加密切(R5=0.398)。结论大肠癌组织中VEGF的表达与骨髓中癌细胞的微转移密切相关,二者联合检测可做为指导临床治疗及判断预后的指标。

Objective To study the expression of vascular endothelial growth factor （VEGF） in colorectal carcinoma and its relationship with micrometastasis of bone marrow. Methods The expression of VEGF protein in colorectal carcinoma tissues obtained from 70 patients who underwent radical resection was detected by immunohistochemistry. The tumor cells were detected by expression of EMA in bone marrow. Results VEGF protein was expressed in 52.9% of patients. Its expression was more frequently detected in tumors with bone marrow micrometastasis than those without （ P 〈 0, 01 ）. Conclusion The expression of VEGF is closely related to bone marrow micrometastasis of colorectal carcinoma. Examination of VEGF and bone marrow micrometastasis has clinical significance in selecting therapeutical strategy and assessing the prognosis of the disease.