血管紧张素转换酶基因及CD14基因多态性与变应性鼻炎的关系

The Correlation of ACE Gene Polymorphism and -159C/T Polymorphism of CD14 Gene Promoter with Allergic Rhinitis

目的:通过对血管紧张素转换酶(ACE)基因插入/缺失的多态性及CD14/-159C/T基因的多态性分析,探讨其在变应性鼻炎发病中的作用。方法:采用聚合酶链反应(PCR)扩增及限制性内切酶片段多态性(PCR-RFLP)分析方法测定60例变应性鼻炎患者和40例健康对照者的ACE基因型和CD14/-159C/T基因型的分布情况,并采用相关统计学方法对结果进行分析。结果:(1)ACE等位基因I、D频率在变应性鼻炎组和对照组的分布差异有统计学意义(χ2=17.37,P<0.01)。等位基因D与变应性鼻炎高度相关(OR=3.46,95%CI=1.92￣6.23,P<0.01)。(2)CD14/-159C/T等位基因C、T频率在变应性鼻炎组和对照组的分布差别具有统计学意义(χ2=14.53,P<0.01),等位基因C与变应性鼻炎高度相关(OR=3.1,95%CI=1.75～5.47,P<0.01)。2组基因型(CC型、CT型、TT型)频率的分布比较差别也有统计学意义(χ2=11.77,P<0.01)。结论:ACE基因多态性与CD14基因的多态性是影响变应性鼻炎的重要候选基因,其中ACE的D等位基因与CD14-159C/T的C等位基因与变应性鼻炎相关。

Objective： To investigate the correlation of angiotensin-converting enzyme （ACE） gene polymorphism and - 159C/T polymorphism of CD14 gene promoter with allergic rhinitis and to approach its pathogenesis for guiding future prophylaxis and therapies. Methods： ACE genotype and CD14 genotype were detected in 60 allergic rhinitis patients and 40 health control by polymerase chain reaction （PCR） and restriction fragment length polymorphism （PCR-RFLP）. A series of statistic were used to analysis the experimental results. Results： （1） There were significant differences in I and D allele frequencies at ACE gene （X^2 = 17.37,P 〈 0.01）. Allele D was associated with allergic rhinitis （OR = 3.46, 95% CI = 1.92-6.23, P 〈 0.01）. （2） There were significant differences in C and T allele frequencies at CD14/-159 locus （X2 = 14.53,P 〈 0.01）. Allele C was associated with allergic rhinitis （OR=3.1, 95%CI = 1.75-5.47, P 〈 0.01）. There was a statistical significance in frequency distribution between two gene types （X^2= 11.77, P 〈 0.01）. Conclusion：ACE gene and CD 14 locus polymorphism may be an important candidate gene for allergic rhinitis. Allele D and allele C are associated with allergic rhinitis.