摘要
目的:探讨正常大鼠与甲状腺功能亢进(甲亢)大鼠脑甲状腺激素受体(TR)mRNA表达差异。方法:将10只实验大鼠随机分2组,每组5只,荧光定量PCR(FQ-PCR)方法检测甲亢大鼠各脑区甲状腺激素受体mRNA表达变化。结果:与正常大鼠相比,甲亢大鼠大脑、小脑、海马、脑干中TRα1mRNA分别下调43%、35%、52%、49%(P<0.01)。小脑中TRα2mRNA无显著性变化(P>0.05),大脑TRα2mRNA下调34%(P<0.05),海马、脑干中TRα2mRNA分别上调67%(P<0.01)、56%(P<0.05)。小脑中TRβ1mRNA无显著性变化(P>0.05),大脑、海马、脑干中TRβ1mRNA分别下调41%(P<0.01)、50%(P<0.05)、46%(P<0.05)。各脑区TRα1与TRα2mRNA比值均下降(P<0.05)。结论:甲亢时各脑区对高甲状腺激素水平敏感性不一致,甲状腺激素受体mRNA异常表达变化可能参与了甲亢时脑功能障碍的发病机制。
Objective： To study the difference of thyroid hormone receptor mRNA expression in normal and hyperthyroid rat brain. Methods： Ten rats were randomly divided into two groups （n=5） including normal control group and hyperthyroid group administrated with thyroid tablets. Thyroid hormone receptor mRNA expressions in various areas of hyperthyroid rat brain were detected by fluorescence quantitation PCR （FQ-PCR）. Results： TRα1 mRNA in hyperthyroid rat decreased 43%, 35%, 52% and 49% in cerebrum, cerebellum, Hippocampus and brain stem respectively compared with control group （P〈 0.01）. TRα2 mRNA remained constant in cerebellum （P 〉 0.05）, decreased 34% in cerebrum （P 〈 0.05） and increased 67% and 56% in hippocampus and brain stem respectively （P 〈 0.01, P 〈 0.05）. TRβ1 mRNA remained constant in cerebellum （P 〉0.05） and decreased 41%, 50% and 46% in cerebrum, hippocampus and brain stem respectively （P 〈 0.01, P 〈 0.05, P 〈 0.05）. TRα1 ： TRα2 ratio decreased in each brain area （P 〈 0.05）. Conclusion： Different brain area has different response to high thyroid hormone level. The abnormal of TR mRNA expression may participate in the pathogenesis of hyperthyroid mental disorder.
出处
《天津医药》
CAS
北大核心
2006年第12期877-879,共3页
Tianjin Medical Journal
基金
天津市自然科学基金资助项目(项目编号:033605711)
