摘要
目的测定过氧乙酸和84消毒液浸泡IVC笼盒消毒灭菌效果。方法测定在不同浓度、不同时间内浸泡IVC笼具的细菌总数及笼盒内空气落下菌。结果与结论0.2%、0.5%、1.0%、2.0%过氧乙酸和84消毒液浸泡IVC笼盒5 min均可达到消毒无菌效果,为了安全保险,本实验室采用0.5%过氧乙酸和0.5%84消毒液浸泡10 min,效果很好,一年多未见污染,裸小鼠质量检测可达SPF级。
Objective To evaluate the disinfection effects of acetic peroxide and 84-disinfectant on individually ventilated cages (IVC). Methods IVC cages were soaked with acetic peroxide or disinfectant-84 for corespondingly fixed time and concentration. The total bacteria and air berne bacteria (koch) in IVC cages were determined. Results and Conclusion IVC cages can be disinfected by beth 0.2% , 0.5%, 1.0%, 2.0% To be safe, we soaked IVC cages with 0.5% acetic peroxide acetic peroxide and disinfectant-84, respectively, for 5 minutes. or disinfectant-84,for 10 minutes, alternatively. The effect is excellent. No pollution has been observed for more than one year. SPF is obtained for nude mice kept in such disinfected IVC cages.
出处
《中国比较医学杂志》
CAS
2006年第12期757-759,共3页
Chinese Journal of Comparative Medicine
关键词
过氧乙酸
84消毒液
IVC
灭菌
Acetic peroxide
disinfectant-84
IVC
Disinfection