特异性HSV抗体检测在生殖器疱疹诊断中的重要性分析

Reseach of Clinical and Laboratory Diagnosis of Herpes Simplex Virus Ⅰ and Ⅱ Types Infection

目的探讨生殖器疱疹(GH)患者HSV-Ⅰ、Ⅱ型IgG、IgM抗体检测的临床意义,评价其对GH诊断的重要性和实用性。方法应用酶联免疫吸附试验(ELISA)对生殖器部位有皮损者检测HSV的抗原及IgG、IgM抗体。结果男性患者有生殖器皮损者215例,HSV抗原阳性率45.58%(98/215),抗体阳性率98.14%(211/215)。女性患者有生殖器皮损者193例,HSV抗原阳性率为38.86%(75/193),抗体阳性率99.48%(192/193)。生殖器有皮损者HSV抗体阳性率均明显高于抗原阳性率。男女IgMⅠ型阳性率42.37%(100/236),提示HSV-Ⅰ所致GH明显增加。结论对生殖器皮损时间短的进行HSV抗原检测,及对生殖器皮损时间长或反复者进行抗体检测是判断HSV感染的检测方法。IgM抗体检测对有生殖器皮损但抗原检测阴性,及对无皮损排毒期具有辅助诊断意义。

Objective To explore the clinical significance of detecting herpes simplex virus （HSV） Ⅰ and Ⅱ types IgG, IgM antibodies in Genital herps （GH） patients, and to evaluate practicability and necessity of the laboratory assay. Methods The Enzyme linked immunosorbent assay （ELISA） detected HSV antigen and IgG, IgM antibodies in patients with GH symptoms. Results The positive rates of HSV antigen and antibodies respectively were 45.58%（98/215）, 98.14% （211/215） in 215 patients. The positive rate of HSV antigen respectively is 30.63% （102/333）, 35.31%（149/422） in 193 patients with GH symptoms. The positive rate of HSV antibody is higher than that of HSV antigen in patients with GH symptoms markedly. The positive rate of Ⅰ IgM in male and female 236 patients is 42.37%（100/236）, showing a marked increase in GH by HSV-Ⅰ. Conclusion It is the detection method to detect HSV antigen for patients with GH symptoms and shortly, to detect HSV antibodies for patients without GH cutaneous lesion and long or repoafly. The detection of HSV IgM has auxiliary diagnostic significance for patients with GH cutaneous lesion and negative HSV antigen, and ones without GH cutaneous lesion but excreting HSV.