兔眼孔源性视网膜脱离手术复位后视功能以及超微结构的变化

Visual Function and Ultrastructure of Reattached Retina after Rhegmatogenous Retinal Detachment Surgery in Colored Rabbit Models

目的对比研究兔眼视网膜脱离后选择不同时期手术复位视功能的变化情况,为临床手术时机的选择及预测术后视功能的恢复情况提供理论与实验依据。方法利用家兔制备孔源性视网膜脱离模型,成模后1 d、7 d1、4 d时经手术达解剖复位,采用多焦视网膜电流图检测复位后视网膜的功能,数据处理应用SPSS软件。制备组织病理学切片。结果多焦视网膜电流图显示1 d、7 d、14 d的RRD手术复位后P1波平均象限反应密度(QAP1,nV/deg2),P1波幅值(AP1,μV),N1波幅值(AN1,μV),P1波潜伏期(TP1,ms),N1波潜伏期(TN1,ms)各项数值差异有显著性(P<0.05)。光镜电镜显示视网膜脱离复位后组织病理学改变。结论视网膜脱离手术复位后视网膜细胞功能的恢复与脱离时间有明显的负相关性;多焦视网膜电流图对于局部视网膜功能的评价有重要意义;组织病理学研究提供了解释视功能变化的重要依据。

Objective To compare the therapeutic effects of retinal detachment operation performed at different stages on the visual function, and to provide theoretical and experimental evidence for choosing the best operation time and recovery of visual function. Metlmds 15 colored rabbits were used for rhegmatogenous retinal detachment. The animals were for retinal reattaehment after 1, 7 and 14 days, respectively. Multifoeal eletroretinography was performed and tissue samples were taken for 1/ght and electron microscopic examinations. Results The average response densities of P-wave at the first day detached retina after rhegmatogenous retinal detachment were significantly longer than those at seventh and fourteenth days, respectively, and the lateneies of N-wave and P-wave were significantly smaller than those in other groups. Both the light and electron microscopic examinations depicted morphological alterations in the rhegmatogenous retinal detachment eyes followed by reattaehment surgery. Conclusion The results demonstrated that there is a negative correlation between the recovery of retinal cells function after reattaehment surgery and the post-operation time after rhegmatogenous retinal detachment. Multifocal eletroretinography may have significant value in evaluation of topical visual function of the retina. Histopathologieal examination may provide important evidence for explaination of the alterations of visual function.