雷洛昔芬对大鼠成骨细胞分泌细胞因子的影响

Effects of Raloxifene on cytokine secretion in osteoblasts in rats

目的初步探讨雷洛昔芬对成骨细胞自分泌和旁分泌的作用机制。方法采用新生大鼠颅骨来源酶消化法体外培养成骨细胞，在培养液中加入不同浓度雷洛昔芬（0、10^-8、10^-7、10^-6mol／L），在共同条件下培养。应用酶联免疫法（EusA）测定细胞培养上清液中IL-1β、IL-6和TNF-α水平。结果雷洛昔芬（10^-8mol／L组和10^-7mol／L组）的IL-1β、IL-6值低于对照组，雷洛昔芬各组TNF-α值均高于对照组，3项测试指标在0～24h和24～48h组间比较无显著性差异。结论适当浓度下雷洛昔芬（10^-8M，10＆-7M）抑制大鼠成骨细胞分泌IL-1β和IL-6，雷洛昔芬能够通过调节成骨细胞的自分泌及旁分泌影响破骨细胞的功能。雷洛昔芬促进大鼠成骨细胞分泌TNF-α，雷洛昔芬在体内对TNF-α分泌与调节的作用待进一步研究。

Objective To explore the mechanisms of the role of raloxifene on osteoblastic autocrine and paracrine. Methods Osteoblasts were isolated from the ealvaria of neonatal rats through trypsin and eollagenase digestion. Different concentrations of raloxifene （0,10^-8 M, 10^-7 M and 10^-6 mol/L） were added into the culture medium. The levels of IL-1β, IL-6 and TNF-α in the cultures media of rat nsteoblasts were measured by ELISA.Results The concentration of IL-1β and IL-6 in the groups with raloxifene, at 10^-8 M and 10^-7 M, were lower than those of the control, and the concentration of TNF-α in all raloxifene groups were higher than that of the control except for the raloxifene groups, at 10^-6 M, in the 0 - 24 hours. No significant difference was shown between the 0 - 24 hours and 24 - 48 hours＇ production of cytokines among any raloxifene group with the control group. Conclusions Certain concentrations of raloxifene （10^-8 M, 10^-7 M）could inhibit IL-1β and IL-6 secretion in rat osteoblasts and was able to affect the function of osteoclasts through modulating the autocrine and paracrine function of osteoblasts. Raloxifene can increase TNF-α production in rat osteoblasts iu vitro, however, more study is pending on its mechanism.