大豆苷元磺酸钠对维甲酸诱导大鼠骨质疏松的保护作用

Protective effect of sodium daidzein sulfonateon on osteoporosis induced by retinoic acid in rats

目的研究大豆苷元磺酸钠对维甲酸诱导大鼠骨质疏松的保护作用。方法以70mg/kg维甲酸诱导大鼠形成骨质疏松模型。自实验开始当天下午各组大鼠依次经口给予以下药物阴性对照组(Control)、病理模型组(Model)0.5%CMC-Na溶液5ml/kg;骨疏康组(Gsk)4g/kg;大豆苷元磺酸钠高、中、低剂量组(H-SDS300mg/kg;M-SDS150mg/kg;L-SDS75mg/kg)。连续给药5周。结果大豆苷元磺酸钠连续给药5周后,高、中剂量组骨钙素(s-BGP)均与对照组水平相当,并且可明显增加股骨骨密度;高剂量组灰重系数和骨钙含量均较模型组显著增加。高、中、低3个剂量组骨小梁形态结构趋于完整,骨髓腔呈圆形或卵圆形且腔隙较小;骨小梁面积(TS%)和平均骨小梁宽度(MTT)明显增加;骨表面平整光滑。结论大豆苷元磺酸钠对维甲酸诱导的骨质疏松大鼠的骨小梁具有保护作用,能防治维甲酸诱导的大鼠骨质疏松症。

Objective To study the protective effects of Sodium Daidzein Sulfonateon （SDS） on osteoporosis induced by retinoic acid （RA） in rats. Methods The osteoporosis model of rats was induced by administration of retinoic acid at the dose of 70 mk/kg for 15 days. The rats were treated for 35 days with nothing （ the negative control）, vehicle （the model group： 5 ml/kg）, Gushukang （Gsk： 4 g/kg）, or SDS at high, medium and low dose （H-SDS： 300 mk/kg, M-SDS：150 mldkg, L-SDS：75 mg/kg） .Results The serum levels of osteocalcin in the H- SDS and M-SDS groups were comparable with that of the control after 35 days of medication. Both Wash/Wfemur and femoral BMD were significantly increased in H-SDS and M-SDS group compared to the model group. Administration of SDS prevented the change of trabecular bone microarchitecture induced by RA, increased the trabecular bone area and trabecular thickness, while decreased the trabecular separation, Conclusions SDS administration prevents BMD decrease induced by RA, and exhibits a moderate protective effect on microarchitecture of trabecular bone.