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卵子冷冻在体外受精-胚胎移植中的实验研究

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摘要 目的研究人类不同成熟期卵子冷冻与复苏的实验条件,观察慢冻速融后卵子形态、存活状况,探讨卵子冷冻的方法。方法收集自愿捐赠获得的辅助生殖周期中的卵母细胞进行慢冻速融,观察成熟卵子(MII)和未成熟卵子(MI+GV)的形态学变化以及未成熟卵冻融后体外成熟情况。结果本实验冻存卵子47例,存活18例,含13例成熟卵子(MII)和5例未成熟卵子(MI+GV),存活率38.30%。结论本法冻融卵子有部分卵子获得短期存活,为今后卵子冷冻研究提供了一定的实验依据。
出处 《实用临床医学(江西)》 CAS 2006年第12期109-110,共2页 Practical Clinical Medicine
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参考文献10

  • 1PORCU E,FABBRI R,DAMIANO G,et al.Clinical Experience and Application of Oocyte Cryop2 Reservation[J].Mo1 Cell Endocrinol,2000,169(1/2):33-37.
  • 2PORCU E.Oocyte Freezing[J].Semin Reprod Med,2001,19(3):221-230.
  • 3李梅,陈子江,李媛,赵力新,高选,马水英.慢速冷冻对人成熟卵与未成熟卵发育潜能的影响[J].实用妇产科杂志,2003,19(3):147-149. 被引量:5
  • 4李媛,陈子江,冯云,高士友.人类胚胎冻存技术的初步研究[J].山东医科大学学报,1999,37(4):308-310. 被引量:4
  • 5FABBRI R,PORCU E,ARSELLA T,et al.Human Oocyte Cryopreservation:New Perspectives Regarding Oocyte Survival[J].Hum Reprod,2001,16(3):411-416.
  • 6陈子江,李梅,李媛,赵力新,唐蓉,高芹.人类MⅡ期卵冷冻与复苏的初步实验研究[J].现代妇产科进展,2003,12(1):4-7. 被引量:6
  • 7STACHECKI J J,COHEN J,WILLADSEN S,et al.Detrimental Effects of Sodium During Mouse Oocyte Cryopreser-vation[J].Biology of Reprod,1998,59(2):395-400.
  • 8PARK S E,SON W Y,LEE S H,et al.Chromosome and Spindle Configurations of Human Oocytes Matured in Vitro After Cryopreservation at the Germinal Vesicle Stage[J].Fertil Steril,1997,68(5):920-926.
  • 9孙伟,宁玻.卵子冷冻技术在IVF-ET中的应用[J].中国优生与遗传杂志,2003,11(1):8-9. 被引量:2
  • 10FABBRI R,PORCU E,MARSELLA T,et al.Technical Aspects of Oocyte Cryopreservation[J].Mol Cell Endocrinol,2000,169(1/2):39-42.

二级参考文献10

  • 1陈子江,冯云,苏应宽.宫腔配子移植术44例报告[J].中华妇产科杂志,1996,31(9):563-564. 被引量:8
  • 2Fabbri R, Porcu E, Matsella T, et al. Human oocyte cryopreservation: new perspectives regarding oocyte survival. Hum Reprod,2001,16(3) :411-416.
  • 3Natalie AC, Catherine MHC, David AG, et al. A novel system for in vitro maturation of human oocytes. Fertil Steril,2001,75(6) : 1185-1193.
  • 4Goud A,Goud P, Qian C,et al. Cryopreservation of human germinal vasical stage and in vitro matured MⅡ oocytes: influence of cryopreservation media on the survival, fertilization,and early cleavage divisions. Fertil Steril,2000,74(3) :487-494.
  • 5Park SE,Son WY,Lee SH, et al. Chromosome and spindle oonfigurations of human oocytes matured in vitro after cryopreservation at the germinal vesicle stage. Fertil Steril, 1997,68(5) :920-926.
  • 6Nogueira D, Staessen C, Van de Velde H,et al. Nuclear status and cytogenetics of embryos derived from in vitro-matured oocytes. Fertil Steril, 2000,74(2) :295-298.
  • 7Shinji Murayama,Shuji Yamano,Tsuzuki Kobayashi,Hiromi Ishikawa,Kenji Kunikata,Toshihiro Aono. Successful freezing of unfertilized mouse oocytes and effect of cocultures in oviducts on development of in vitro fertilized embryos after thawing[J] 1994,Journal of Assisted Reproduction and Genetics(3):156~161
  • 8李媛,陈子江,冯云,高士友.人类胚胎冻存技术的初步研究[J].山东医科大学学报,1999,37(4):308-310. 被引量:4
  • 9孙梅,冯云,陈子江.取卵数目与卵子成熟对IVF-ET的影响[J].生殖与避孕,2000,20(3):184-186. 被引量:16
  • 10李媛,陈子江,赵力新,李梅,唐蓉,许成岩.未成熟卵母细胞体外成熟后体外授精-胚胎移植成功分娩1例[J].中华男科学杂志,2002,8(1):70-71. 被引量:11

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