摘要
目的研究中国南海棕环海参的化学成分。方法采用稻瘟霉模型生物活性追踪方法,应用多种色谱技术分离,根据波谱解析和化学手段鉴定化合物的结构。结果从棕环海参正丁醇萃取物中分离鉴定了3个三萜皂苷:pervicoside C(Ⅰ),holothurin A(Ⅱ)和DS-holoturin B(Ⅲ);从氯仿萃取物中分离鉴定了4个化合物:尿嘧啶(Ⅳ),胸腺嘧啶(Ⅴ),胸腺嘧啶脱氧核苷(Ⅵ)和尿嘧啶脱氧核苷(Ⅶ)。结论所有化合物均为首次从该种海参中获得,化合物I为新天然产物,Ⅰ和Ⅱ具有诱导稻瘟霉菌丝变形活性和肿瘤细胞毒性。
Objective To study the chemical constituents of the sea cucumber Holothuria fuscocinerea collected from the South China Sea. Methods The compounds were isolated by Pyricularia oryzae bioassay-guided fractionation method in combination with extraction and partition as well as multi-chromatography. Their structures were determined on the hasis of spectral analysis and chemical evidence. Results Three triterpene glycosides were isolated from the n-BuOH extract and identified as： pervicoside C （Ⅰ）, holothurin A （Ⅱ） and DS-holoturin B （Ⅲ）, Four compounds [uracil （Ⅳ）, thymine （Ⅴ）, thymidine （Ⅵ） and 2-deoxyuridine （Ⅶ）] were obtained from the CHCla extract. Conclusion All the compounds were isolated from this sea cucumber for the first time while Compound Ⅰ was first obtained as pure compound. Compound Ⅰ and Ⅱ were active compounds causing morphological abnormality of P. oryzae mycelia and exhibited cytotoxicity against two cancer cell lines .
出处
《中国海洋药物》
CAS
CSCD
2006年第6期7-13,共7页
Chinese Journal of Marine Drugs
关键词
海参
棕环海参
三萜皂苷
稻瘟霉
细胞毒性
sea cucumber
Holothuria fuscocinerea
triterpene glycosides
Pyricularia oryzae
cytotoxicity
