慢性低氧高二氧化碳大鼠支气管骨桥蛋白及其基因的表达

Expression of osteopontin protein and its gene in the bronchi of chronic hypoxic hypercapnic rats

目的研究骨桥蛋白(OPN)基因和蛋白在慢性低氧高二氧化碳大鼠的支气管表达情况。方法采用免疫组化和原位杂交技术检测大鼠支气管OPN蛋白和mRNA表达情况。结果OPN蛋白和mRNA在低氧高二氧化碳组大鼠支气管上皮细胞及平滑肌细胞中广泛表达,与对照组比较均有显著性差异(P<0.01)。结论慢性低氧高二氧化碳诱导了大鼠支气管骨桥蛋白的表达。

Objective To study the role of the gene and protein of expression of osteopontin （OPN） in the bronchi of chronic hypoxic hypereapnic rats, Methods OPN gone and protein expression in bronchi were detected by in situ hybridization and immunohistochemistry respectively. Results OPN gene and protein widely expressed in epithella and smooth muscle cells of bronchi in rats of hypoxic hyperoapnia group having significant difference with those of control （P 〈 0. 01 ）. Conclusions Expression of OPN gene and protein in bronchi of rats may be induced by chronic hypoxic hypercapnia.