血红素氧合酶-1对动脉粥样硬化大鼠MDA CD68及MMP-9的影响

Effects of heme oxygenase-1 on MDA CD68 and MMP-9 in atherosclerotic rat

目的:探讨血红素氧合酶-1(HO-1)对动脉粥样硬化斑块稳定性的影响及其机制。方法:30只雄性SD大鼠随机分为正常对照组、模型组及氯血红素组,分别接受正常饮食、高胆固醇饮食及高胆固醇饮食加氯血红素腹腔注射共3个月。实验结束后取血测血清丙二醛(MDA)水平,免疫组化法检测斑块内HO-1、CD68及金属蛋白酶-9(MMP-9)的表达。结果:模型组及氯血红素组与对照组比较,MDA、HO-1、CD68及MMP-9升高(P<0·05)。氯血红素组与模型组比较,主动脉内膜斑块面积占内膜面积比(RAAPI)显著减少(P<0·01),HO-1升高(P<0·01),MDA、CD68及MMP-9显著降低(P<0·05)。结论:HO-1可能通过减少MDA、CD68及MMP-9的表达,抑制脂质过氧化及炎症反应,下调纤维帽降解因素,从而达到稳定斑块的作用。

Objective： To explore the mechanism and the effect of heine oxygenase-1 （HO-1） on atherosclerotic plaque stability. Method：Thirty male SD rats were randomly divided into three groups： control group, model group and heroin group. They were respectively fed with one of three diets, a reference diet, a high cholesterol supplemented diet and a high cholesterol supplemented with heroin. After three months, serum concentrations of MDA of each group were measured. The expression of HO-1, CD68 and MMP-9 in plaque were measured by means of immuneohistochemistry. Result： Compared to control group, MDA, HO-1, CD68 and MMP-9 of the model group and the heroin group were increased significantly （ P 〈 0.05）. Compared to the model group, HO-1 of the heroin group was increased significantly （P 〈 0.01）, while the RAAPI, MDA, CD68 and MMP-9 decreased （P 〈 0.05）. Conclusion：HO-1 might play an important role in stabilizing atherosclerotic plaque through its antioxidative and antiinflammatory effects.