利用SAS软件优化桑黄发酵培养基

SAS Application in Studies on Medium Optimization of Phellinus Linteus

利用SAS软件对桑黄菌的发酵培养基进行研究。首先,运用Plackett-Burman设计法筛选出影响粗多糖产量的主要因素,继而采用中心组合设计及响应面分析确定主要影响因子的最佳浓度。通过试验发现,当豆饼粉3.7%、玉米浆0.6%、CaCl20.27%时,胞外粗多糖产量达到最大值6.78g/L,较优化前的5.24g/L提高了29.4%。另外还对其胞外多糖含量、分子质量进行了测定。

SAS system was used to optimize the medium components for crude exopolysaccharides production by Phellinus linteus. Firstly, the prime factors affecting crude exopolysaccharides yield were selected by means of Plackett-Burman design; Then, a central composite design was used to optimize the prime factors and the results were shown in response surface polts. When the medium comprised soybean cake 3.7 %, corn steep powder 0.6 %, CaCl20.27 %, the crude exopolysaccharides yield could reach 6.78 g/L, Which was 29.4 % higher than the control. In addition, the content and the molecular weight of exopolysaccharides were determined.